Rates the HMW adiponectin levels; overall, among the women with PCOS
Rates the HMW adiponectin levels; overall, among the women with PCOS, both were significantly decreased in the plasma (White Box) and the FF (Black box) (P < 0.001 and P = 0.002, respectively) in comparison to controls. Compared with the plasma HMW adiponectin levels (White Box), the HMW adiponectin levels in FF (Black box) were significantly lower in the PCOS group and the control group (P = 0.007 and P = 0.002, respectively). The data are the means ?SEM. The P-value for the Wilcoxon and Mann hitney U tests for the difference between the two groups.Tao et al. Reproductive Biology and Endocrinology 2013, 11:99 http://www.rbej.com/content/11/1/Page 5 ofTable 2 The correlation between HOMA-IR and total and HMW adiponectin and the number of follicles and larger follicular diameters among the women with and without PCOS undergoing IVFVariable Number of follicles Larger follicular diameters Total adiponectin in FF HMW adiponectin in FF Total adiponectin in serum HMW adiponectin in serum -0.784 -0.445 -0.759 -0.726 -0.586 -0.763 HOMA-IR Correlation P <0.001 0.049 <0.0001 <0.0001 0.007 <0.P-value PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27797473 for Spearman’s correlation tests. HMW – high molecular weight, FF-follicular fluid, HOMA-IR – homeostasis model assessment – insulin resistance index.follicles had a strong negative relationship with the HMW adiponectin levels (r = -0.629, P = 0.003) and the total adiponectin levels (r = -0.679, P = 0.001) in the FF.Correlation between the levels of the total and the HMW adiponectin in the serum and the number of follicles and larger follicular diametersAs shown in Figure 3, considering all the women with and without PCOS undergoing controlled ovarian hyperstimulation, a strong negative linear correlation was observed between the number of follicles and the HMW adiponectin levels (r = -0.574, P = 0.008) and the total adiponectin levels (r = -0.522, P = 0.018) in the serum. We did not find that the total adiponectin and the HMW adiponectin levels in the serum have strong linear relationships with larger follicular diameters (P > 0.05 for both).Discussion The major finding of this study is the demonstration that lower HMW adiponectin and total adiponectin levels in FF and serum was an important feature in gonadotropin stimulated PCOS patients. Decreased HMW adiponectin levels in the FF was associated with decreased larger follicular diameters in normovulatory and PCOS women under PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25957400 IVF, and this association is independent of overall adiposity. The HMW adiponectin provided a stronger relationship with larger follicular diameters than the total adiponectin among all the women under IVF. Our data suggested that IR, estimated by HOMA-IR, at least partially predicts a reflection of ovarian stimulation in IVF patients. Findings from previous animal studies show that adiponectin provokes the expression of genes associated with peri-ovulatory remodelling of the ovarian follicle and may be involved in the regulation of follicle maturation and ovulation [12,21,22]. Multiple endocrine and intraovarian paracrine interactions may influence theintrafollicular microenvironment for appropriate oocyte development. The CPI-455 supplier concentrations of adipokines in FF correlate with folliculogenesis and oocytes more than with implantation and embryonic development. Recent studies showed that endocrine/paracrine abnormalities and metabolic dysfunction impaired folliculogenesis in PCOS patients and determined the outcome of assisted reproductive technologies (ART) [23]. The findings of our.