, and harm to the template strand produce challenges for full and accurate DNA replication. The 79831-76-8 replication tension response maintains genome integrity through sensing and overcoming these challenges by…
ice-cold PBS. Cells were lysed by incubating for 5 mins in cold lysis buffer. Resulting nuclei were washed in MNase buffer. Chromatin was subjected to micrococcal nuclease digestion, with pre-determined…
al levels, it is likely that the high concentrations of the proteins may force interactions that would not occur at physiological levels of expression. To diminish that concern, we examined…
cells from other sources could inhibit myofibroblast differentiation of lung fibroblasts. We co-cultured lung fibroblasts with either primary human neonatal epidermal keratinocytes or the human lung alveolar epithelial cell line,…
ropriate intervals, all the receptor liquid was withdrawn from the glass cells and an equal volume of the same receptor liquid was added to maintain a constant volume. The amount…
riments to monitor LC3 turnover confirmed that limonene enhanced autophagic flux. In fact, limonene induced a dramatic but transient increase in LC3II levels a finding consistent with the notion that…
5uC for 10 s, 60uC for 30 s; 72uC PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/19718802 immunoprecipitates were washed four times with RIPA buffer and the immunoprecipitated proteins were analyzed by western blot using ubiqutin…
ns and there is an influx of Ca2+ ions into the mitochondria. The accumulation ruptures the outer mitochondrial membrane and Rhodamine-123 leaks out from the mitochondria into the cytosol. Rhodamine-123…
ical line extends from a promoter region to note a corresponding position in the respective lower charts. Red circles and blue rectangles denote the Up/Lo values of active and repressed…
ed and cloned into the pIRES-puro Glue vector downstream of the tandem affinity tags. The p3FLAGCMV14 plasmid with human cDNA for TRAFD1 was a gift from Dr. Akihiko Yoshimura, Keio…