eal System, was described with AmberParm99 force field. Considering that the DFT method usually underestimates the energy barriers of methyl transfer, we also used the MP2 method with 6-31G basis…
cted by NAE can form thioester conjugates with NAE and Ubc12. We synthesized peptides corresponding to the last seven residues of the UB variants N1, N7, N11, N21, N26, N27,…
reased expression of KLK5 and KLK7 protein in EBS-DM cell lines determined in 48-h-conditioned cell culture supernatant by western blot. Student's t-test was performed with p values: 0.01, 0.005. doi:10.1371/journal.pone.0070123.g001…
est that bed bugs are an unlikely vector of Bartonella spp. However, as Bartonella are emerging pathogens, adaptation to new hosts in conjunction with an expanding range of vectors BIRB-796…
g African Americans with heart failure, greater European ancestry was correlated with higher serum aldosterone concentrations, and greater West African ancestry was correlated with lower aldosterone levels. These finding are…
demonstrate the functional importance of Sp1 in vivo using our mouse model for PHPV. Regrettably, Sp12/2 mice display an embryonic lethal phenotype at E11.5, before primary vitreous development. 17429684 Tissue…
idase for 30 min at 37uC. Immunoreactivity was visualized with 0.05% diaminobenzidine . Negative controls received the same treatments except that primary antibodies were omitted, and showed no specific staining.…
e to DNA damage. Previous studies have indicated that p53 is another important substrate of RSK2 for chromatin remodeling and regulation of gene expression. RSK2 activates and phosphorylates p53 in…
ate for the inner primer, and the thermocycler conditions were as follows: 30 cycles at 95uC for 30 sec, 58uC for 30 sec, and 72uC for 1 min. To clone…
of Recombinant 19271755 Proteins of MPO by ELISA Highly purified recombinant MPO proteins were reconstituted to 10 mg/ml with coating buffer. A 100 ml portion of the mixture was then…