arts of this protein, a N-linked glycosylation site presented at residues 131 along with conserved consensus casein kinase II phosphorylation sites at residues 31, 52, 71, 110 and 114 predicted by the PHD algorithms were observed . To study the phylogenetic relationship among the predicted amino acid sequence of Mj-FAR-1 and other nematodes FAR, a BLASTp search through available online databases was conducted using the amino acid sequence of Gp-FAR-1 of G. pallida as the query using ClustalW in MEGA5.0 software. A phylogenetic inference of the FAR proteins is presented in Fig. 1B, showing five main clusters A to E, represented by: FAR proteins of the free-living nematode Caenorhabditis elegans, animal-parasitic nematodes, root-knot nematodes, endo-migratory plant-parasitic nematodes and cyst nematodes. Protein alignment of Mj-FAR-1 against Ce-FAR members of clade A determined a 41%, 39% and 31% sequence identity to CeFAR-1, Ce-FAR-2 and Ce-FAR-6 of C. elegans, respectively. As expected, Mj-FAR-1 grouped closely with those FARs belong to other parasitic nematodes, exhibiting 45%, 52%, 46% and 47% sequence identity with FARs of animal-parasitic nematodes, such as Ascaris suum As-FAR, Toxocara canis Tc-FAR, including the human-parasitic nematodes Onchocera volvulus Ov-FAR-1 and Wuchereria bancrofti WbFAR, respectively. The sequence similarity observed against cyst nematode species, represented by G. pallida Gp-FAR-1, G. GW 501516 web rostochiensis Gr-FAR, Heterodera glycines Hg-FAR and H. schachtii Hs-FAR, vary between 64% to 68% against Mj-FAR-1. The sequence identity observed between Mj-FAR-1 
and the FAR of migratory endoparasites nematodes increased from 73% to 75%, related to Pratylenchus vulnus 26646986 Pv-FAR and Radopholus similis Rs-FAR, respectively. The highest predicted amino acid identity were observed among Meloidogyne spp., with 98% sequence identity between Mj-FAR-1 and M. incognita MiFAR and M. arenaria Ma-FAR, and 90% identity with M. hapla Mh-FAR. Interestingly, the sequence identity between Mj-FAR-1 and M. chitwoodi Mc-FAR presented the lowest value of 74%. Expression pattern of mj-far-1 gene during nematode development The expression dynamics of mj-far-1 of M. javanica for nonparasitic and parasitic stages was quantified using quantitative real-time reverse transcription-PCR. To assess the mjfar-1 gene expression, equivalent RNA amounts were analyzed for different nematode stages: eggs and pre-parasitic J2s; and within tomato roots, the migratory parasitic J2s, J2s and J3/J4 juveniles and mature females. RNA extracted from non-infected roots was used as a negative control; while transcript levels were normalized for each sample with the geometric mean of the expression of two selected housekeeping genes 18S rRNA and EF-1a. The lowest level of mj-far-1 transcripts were detected within eggs, followed by a marked increase of 18 fold within pre-parasitic J2s, suggesting a strong induction of mj-far-1 transcripts just before the infection process of freshly hatched pre-parasitic J2s. Within the first hours after root infection a relative increase in mj-far-1 transcripts was consistently observed in nematode juveniles collected at 6 h to 48 h after infection. Expression level of mj-far-1 in sedentary juveniles collected at 15 DAI, and in adult females, showed some reduction during nematode development. The detectable levels 26028783 of this gene Mj-FAR-1 Induces Host Susceptibility to RKN transcripts throughout infection suggest a potential role of Mj-FAR d