Ry of Ukraine had started in 600 Th years final century from Crimea. Presently Ambrosia is registered in 234 regions of Ukraine including the Central Element, the collecting region are 1,328,377,863 ha. In Western Ukraine it appeared far more than 20 years ago and its location escalating rapidly. Aim To evaluate the options of sensitization to Ambrosia in individuals from Lviv (Western Ukraine) and Kyiv regions (Central Ukraine) and toanalyze the efficacy of AIT with Extract of Ambrosia (Diater Laboratories, Spain). Procedures: 586 individuals aged 58 had been examined in Kyiv 327 (55.8 ) and 259 (44.2 ) in Lviv region. SPT was performed by Extract “Ambrosia”. The o-Methoxycinnamaldehyde Protocol patients were examined to undergo molecular diagnostics applying ImunoCAP (Phadia) to recognize important (n Amb a1) allergen. The SLIT was carried out having a mixture of Ambrosia. Benefits: The prevalence of Ambrosia sensitization diagnosed in 25 (9.six ) persons in the Lviv region. 3 (12.0 ) children moved in the Crimea and 22 (88.0 ) adults was born and have already been living in Western Ukraine. Sensitization to Ambrosia big allergen (n Amb a1) was detected in 23 (92.0 ) persons. Withal, sensitization to Ambrosia (SPT) in individuals in the Kyiv region was determined 2.5 times greater: optimistic SPT have been detected in 80 (24.5 ) patients (28 (35.0 ) youngsters and 52 (65.0 ) adults). The true sensitization has been confirmed in 88.0 of persons. SLIT have been prescribed in the sufferers from both regions. The efficacy of SLIT was assessed by a visual analogue scale (VAS-up). An assessment of SLIT showed that there was a important decrease within the severity of symptoms in the study groups as following 2 years of therapy (94.1 and 94.5 , respectively). Conclusions: A higher level of sensitization to big allergen (n Amb a1) Ambrosia in youngsters and adults in Ukraine results in a considerable increase in allergic pathology. High efficacy with the SLIT gives the possibility of relative handle of the prevalence of severe type of allergic illnesses. Braco-19 Anti-infection Poster Discussion Session I Topic two: The basis of allergenicity P10 Identification of IgEbinding epitopes around the surface of your nonspecific lipid transfer protein Art V three Sabrina Wildner1, Iris Grie er2, Martina Dimuzio2, Eva Vejvar2, Sara Huber2, Michael Hauser2, Adriano Mari3, Mario Schubert2, Brandstetter Hans2, Gabriele Gadermaier2 1 University of Salzburg, Christian Doppler Laboratory for Biosimilar Characterization, Salzburg, Austria; 2University of Salzburg, Department of Molecular Biology, Division of Allergy and Immunology, Salzburg, Austria; three Connected Centers for Molecular Allergology, Rome, Italy Correspondence: Sabrina Wildner [email protected] Clinical Translational Allergy (CTA) 2018, 8(Suppl 1):P10 Background: Pollen of Artemisia vulgaris (mugwort) are a crucial elicitor of allergic reactions in late summer and autumn. Art v 3 is an allergen of mugwort pollen which belongs for the non-specific lipid transfer protein (LTP) family. The aim of the study is always to resolve the structure of Art v three and to determine the structural epitopes of Art v three using murine monoclonal antibodies. Methods: Recombinant non-labeled and double-labeled (13C15N) Art v three.0201 had been expressed in E. coli and purified making use of cation exchange chromatography. The three-dimensional structure of Art v three was solved by X-ray crystallography and resonance assignment was obtained by NMR spectroscopy. Additionally, three Art v 3-specific murine monoclonal IgG antibodies (mAbs) have been made in hybridoma cells a.