Ry of Ukraine had began in 600 Th years final century from Crimea. Presently Ambrosia is registered in 234 regions of Ukraine like the Central Component, the collecting location are 1,328,377,863 ha. In Western Ukraine it appeared additional than 20 years ago and its area rising immediately. Aim To examine the attributes of sensitization to Ambrosia in sufferers from Lviv (Western Ukraine) and Kyiv regions (Central Ukraine) and toanalyze the efficacy of AIT with Extract of Ambrosia (Diater Laboratories, Spain). Strategies: 586 individuals aged 58 have been examined in Kyiv 327 (55.eight ) and 259 (44.2 ) in Lviv area. SPT was performed by Extract “Ambrosia”. The sufferers were examined to undergo molecular diagnostics utilizing ImunoCAP (Phadia) to recognize important (n Amb a1) allergen. The SLIT was carried out using a mixture of Ambrosia. Final results: The prevalence of Ambrosia sensitization diagnosed in 25 (9.6 ) persons within the Lviv area. 3 (12.0 ) PSEM 89S Technical Information youngsters moved from the Crimea and 22 (88.0 ) adults was born and happen to be living in Western Ukraine. Sensitization to Ambrosia important allergen (n Amb a1) was detected in 23 (92.0 ) persons. Withal, sensitization to Ambrosia (SPT) in patients inside the Kyiv area was determined two.5 times greater: positive SPT had been detected in 80 (24.five ) individuals (28 (35.0 ) youngsters and 52 (65.0 ) adults). The correct sensitization has been confirmed in 88.0 of people. SLIT had been prescribed within the individuals from both regions. The efficacy of SLIT was assessed by a visual analogue scale (VAS-up). An assessment of SLIT showed that there was a considerable decrease within the severity of symptoms in the study groups as right after two years of remedy (94.1 and 94.five , respectively). Conclusions: A high level of sensitization to significant allergen (n Amb a1) Ambrosia in children and adults in Ukraine leads to a important raise in allergic pathology. Higher efficacy on the SLIT provides the possibility of relative handle of the prevalence of severe form of allergic diseases. Poster Oxypurinol Protocol Discussion Session I Subject 2: The basis of allergenicity P10 Identification of IgEbinding epitopes around the surface in the nonspecific lipid transfer protein Art V 3 Sabrina Wildner1, Iris Grie er2, Martina Dimuzio2, Eva Vejvar2, Sara Huber2, Michael Hauser2, Adriano Mari3, Mario Schubert2, Brandstetter Hans2, Gabriele Gadermaier2 1 University of Salzburg, Christian Doppler Laboratory for Biosimilar Characterization, Salzburg, Austria; 2University of Salzburg, Department of Molecular Biology, Division of Allergy and Immunology, Salzburg, Austria; 3 Related Centers for Molecular Allergology, Rome, Italy Correspondence: Sabrina Wildner [email protected] Clinical Translational Allergy (CTA) 2018, 8(Suppl 1):P10 Background: Pollen of Artemisia vulgaris (mugwort) are an essential elicitor of allergic reactions in late summer time and autumn. Art v 3 is an allergen of mugwort pollen which belongs for the non-specific lipid transfer protein (LTP) loved ones. The aim from the study will be to resolve the structure of Art v 3 and to recognize the structural epitopes of Art v 3 applying murine monoclonal antibodies. Solutions: Recombinant non-labeled and double-labeled (13C15N) Art v 3.0201 were expressed in E. coli and purified utilizing cation exchange chromatography. The three-dimensional structure of Art v 3 was solved by X-ray crystallography and resonance assignment was obtained by NMR spectroscopy. Furthermore, three Art v 3-specific murine monoclonal IgG antibodies (mAbs) have been produced in hybridoma cells a.