That the CYP710 family is conserved from green algae to larger plants throughout evolution [46] and that the biochemical function might be traced back to plant-fungal divergence but was lost in animals [40]. During evolution, sterol 14-demethylase (CYP51) gene is assumed to have given rise towards the CYP710/CYP61 genes as their function in sterol biosynthesis is downstream of that of CYP51 [40]. CYP51 enzymes are present in plants, fungi and animals synthesizing sterols. Although the phylogeny of P450 monooxygenases is properly researched, only limited phylogenetic facts is offered for CYP710 [28,40]. General, CYP710 enzyme activity and/or gene expression has only been studied in handful of plants, such as A. thaliana [2,14,19], S. lycopersicum [19], Physcomitrella patens [47] and Calotropis procera [48]. As a result, we carried out a phylogenetic evaluation of our studied tomato SlCYP710A11 protein and otherPlants 2021, ten, FOR Plants 2021, 10, x 292 PEER REVIEW10 of 15 10 ofplant CYP710 enzymes (Figure five;five; TableS3). The well-studied AtCYP710A1 (A. thaliana) plant CYP710 enzymes (Figure Table S3). The well-studied AtCYP710A1 (A. thaliana) and SlCYP710A11 (S. lycopersicum) amino acid sequences were applied as queries to mine for and SlCYP710A11 (S. lycopersicum) amino acid sequences have been made use of as queries to mine for plant homologues. 4 hits had been scored inside a. thaliana: Cytochrome P450 proteins 710A1, plant homologues. 4 hits had been scored inside a. thaliana: Cytochrome P450 proteins 710A1, 710A2, 710A3 and 710A4 (NCBI accessions NP_180997.1, NP_180996.1, NP_180451.1 and 710A2, 710A3 and 710A4 (NCBI accessions NP_180997.1, NP_180996.1, NP_180451.1 and NP_180452.1). ItIt is worth mentioning that in a. thaliana both 710A1 and 710A2 can convert NP_180452.1). is worth mentioning that in a. thaliana each 710A1 and 710A2 can convert -sitosterol to stigmasterol -sitosterol to stigmasterol [19]. For Z. mays, two protein sequences were found within the NCBI For Z. mays, two protein sequences had been identified within the PARP7 Inhibitor manufacturer database, from two two distinctive MEK Inhibitor Species studies, one annotated as `uncharacterized protein’ NCBI database, fromdifferent research, 1 annotated as `uncharacterized protein’ and 1 as one particular as CYP710A11 (NP_001307723.1 and PWZ33314.1, respectively). For two proteins and CYP710A11 (NP_001307723.1 and PWZ33314.1, respectively). For G. max, G. max, two had been identified, 1 annotated as CYP710A1 (XP_003542931.1) and a single as CYP710A11 proteins have been identified, 1 annotated as CYP710A1 (XP_003542931.1) and a single as (XP_003546088.1). Only one homologous homologous protein was found in C. sativus CYP710A11 (XP_003546088.1). Only a single protein was located in C. sativus (XP_004134602.1), also annotated as CYP710A11 (Table S3). Since (Table S3). Considering that B. have been sequences within the (XP_004134602.1), also annotated as CYP710A11 B. juncea sequences junceanot presentwere NCBI or UniProt databases, Brassica rapa was used as a close relative. not present within the NCBI or UniProt databases, Brassica rapa was made use of as a close relative.Figure 5. Phylogenetic maximum likelihood tree ofof the CYP710 enzyme loved ones. The tree is rooted at ERG5, that is the Figure five. Phylogenetic maximum likelihood tree the CYP710 enzyme family members. The tree is rooted at ERG5, which can be the Saccharomyces cerevisiae protein from which all CYP710 proteins originated [20]. A number of sequences with the similar plant Saccharomyces cerevisiae protein from which all CYP710 proteins originated [20]. Various sequences of the identical plant species are n.