Ure 1B). Finally, a total of 15 coexpression modules have been constructed (Figure 1C). A total of 860 genes, accounting for 16.62 , had been not assigned to any of these modules. We assigned a color to and counted the amount of genes in each module. There were 863 genes inside the turquoise module, 788 in the blue module, 635 in the brown module, 432 inside the yellow module, 382 inside the green module, 270 inside the red module, 205 within the black module, 192 inside the pink module, 179 NMDA Receptor medchemexpress inFrontiers in Veterinary Science | www.frontiersin.orgJuly 2021 | Volume 8 | ArticleLiu et al.Network for E. tenella Infected Chickenthe magenta module, 134 within the purple module, 76 inside the greenyellow module, 47 within the tan module, 42 inside the salmon module, 37 within the cyan module, and 33 inside the midnight blue module.CK1 Compound infection Status Associated Modules AnalysisTwo modules of yellow and magenta have been drastically linked with the infection status (primary vs. secondary infection) by the module-trait evaluation (Figure 1E). For module yellow, genes have been involved in biosynthetic and metabolism method (nucleoside phosphate, organophosphate, and carbohydrate derivative) (Figure 3A) and metabolism pathways (histidine, tyrosine, drug metabolism-cytochrome P450, tryptophan and phenylalanine) (Figure 3B). The expression degree of Genes in yellow module decreased considerably inside the principal infection over time and elevated within the secondary infection more than time (Figure 3C). The notion networks showed the information of genes in the prime five GO terms and KEGG pathways (Figures 3D,E). The coexpression network for module yellow genes was shown in Figure 3F. Genes in module magenta had been involved in immune response, defense response and actin filaments connected functions (Figure 4A). The expression degree of genes in this module increased significantly in the primary infection over time (Figure 4B). The notion networks showed the facts of genes in the prime 11 GO terms and IRF1, IFNG, and CAPZA1 have been circled because the important genes (Figure 4C) and also identified as hub genes in this module (Figure 4D).Module Stability TestThe module stability showed that module pink, turquoise, blue, purple, green and black were amongst by far the most steady modules (connectivity correlation 0.8). Module cyan displayed the least stability (Figure 1D).Analysis of the Coexpression Module InteractionsWe analyzed the relationships among the 15 coexpression modules. Module eigengenes in this analysis had been defined because the first principal component of a coexpression module matrix. Cluster analysis was performed on these eigengenes (Figure 1E). The connectivity degree of eigengenes was determined to superior comprehend the interactions amongst the coexpression modules. The heatmap in Figure 1E showed the relatedness with the 15 coexpression modules identified by WGCNA, with red indicating close relatedness and blue indicating no relatedness. The results demonstrated that the gene expression of every single module was mutually exclusive, indicating a high degree of scale independence.Coexpression Modules Drastically Correlated With Different Infection StatusTo determine modules related to main and secondary infection, we calculated the correlations between module eigengenes as well as the infection status (Figure 1F). The modules had been chosen utilizing a correlation p-value of 0.05 as a threshold. The genes inside the magenta (R = -0.61, p = 0.001) and yellow (R = 0.51, p = 0.01) modules are substantially positively or negatively correlated towards the infection st.