390/molecules26195757 Academic Editor: Raffaele Pezzani Received: 21 August 2021 Accepted: 16 September 2021 Published: 23 September10 9Trk Source Department of Medicinal Chemist and Pharmacognosy, College of Pharmacy, Qassim University, Qassim 51452, Saudi Arabia; [email protected] Division of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo 11371, Egypt Department of Pharmacology and Toxicology, College of Pharmacy, Qassim University, Qassim 51452, Saudi Arabia; [email protected] (S.A.A.M.); [email protected] (M.S.M.S.) Department of Biochemistry, Faculty of Medicine, Al-Azhar University, Assiut 71524, Egypt Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, JUST, Irbid 22110, Jordan Hospital of Police Academy, Nasr city, Cairo 11765, Egypt; mohamedshawky1974@yahoo Division of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, South Valley University, Qena 83523, Egypt; [email protected] Department of Pharmaceutics, College of Pharmacy, Qassim University, Qassim 51452, Saudi Arabia; [email protected] Department of Clinical Biochemistry, Faculty of Medicine, Umm Al-Qura University, Abdia, Makkah 21955, Saudi Arabia; [email protected] (S.Y.E.); [email protected] (M.Z.E.-R.) Division of Biochemistry, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, Egypt; [email protected] Correspondence: [email protected] (H.A.M.); [email protected] (H.M.E.); [email protected] (R.A.K.); Tel.: +00966566176074 (H.A.M.)Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.Copyright: 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access short article α4β7 Biological Activity distributed below the terms and circumstances on the Inventive Commons Attribution (CC BY) license ( creativecommons.org/licenses/by/ four.0/).Abstract: Sage, Salvia officinalis L., is employed worldwide as an aromatic herb for culinary purposes also as a standard medicinal agent for various ailments. Current investigations exhibited the effects of extended dryings from the herb around the yields, composition, oil good quality, and hepatoprotective too as anti-cancer biological activities on the hydrodistillation-obtained essential oils from the aerial parts of your plant. The crucial oils’ yields, compositions, and biological activities levels on the fresh and differently timed and room-temperature dried herbs differed drastically. The lowest yields in the vital oil have been obtained from the fresh herbs (FH, 631 mg, 0.16 ), although the highest yield was obtained in the two-week dried herbs (2WDH, 1102 mg, 0.28 ). A notable reduce in monoterpenes, with increment in the sesquiterpene constituents, was observed for the FH-based necessary oil as compared to all the other batches on the important oils obtained in the differenttimed dried herbs. Furthermore, characteristic chemotypic constituents of sage, i.e., -pinene, camphene, -pinene, myrcene, 1, 8-cineole, -thujone, and camphor, had been present in drastically greater proportions in each of the dried herbs’ vital oils as when compared with the FH-based critical oil. The in vivo hepatoprotective activity demonstrated substantial reductions inside the levels of AST, ALT, and ALP, as well as a significant raise in the total protein (p 0.05) contents level, as in comparison with the acetaminophen (AAP) administered experimental group of rats. A important reduction (p 0.05) in the ALT level was demonstrated by the 4W