Intermediates, and inhibit other oxidation reactions, and they do this by
Intermediates, and inhibit other oxidation reactions, and they do that by being oxidized themselves [17-19]. Higher CCR3 Antagonist Accession phenolic content material have been usually correlated with high radical scavenging activity [20]. Choo et al. discovered that H. polyrhizus and H. undatus had great antioxidant properties, due to the fact of higher content material of polyphenols [2]. Furthermore, polyphenols can be extracted by supercritical carbon dioxide extraction [21]. Hence, antioxidant activities on the pitaya peel extracts were most probably because of the presence of polyphenols, which possess the hydrogendonor ability to scavenge the totally free radicals. However, the polyphenols weren’t detected by GC-MS. Studies with the content material of polyphenols in the extracts are at the moment underway.Figure 3 Impact of H. undatus extract on proliferation of cancer cells.Figure 4 Cost-free radical scavenging properties of pitaya peel extracts.Luo et al. Chemistry Central Journal 2014, 8:1 5 ofConclusions In summary, the composition of supercritical carbon dioxide extracts of pitaya (H. polyrhizus and H. undatus) peel has been analyzed by GC-MS, and their cytotoxic and antioxidant activity were investigated. The predominant constituents of H. polyrhizus extract had been amyrin (15.87 ), -amyrin (13.90 ), octacosane (12.2 ), -sitosterol (9.35 ), whereas H. undatus have been -amyrin (23.39 ), -sitosterol (19.32 ), and octadecane (9.25 ). The two extracts showed a wild range of cytotoxic activities against PC3, Bcap-37, and MGC-803 cells, and it was located that -amyrin, -sitosterol, and stigmast-4en-3-one, the key elements, have been responsible for their activities. Additionally, they had some DPPH radical scavenging activities, with IC50 values of 0.83 and 0.91 mg/mL, respectively. There’s a trend to discover cytotoxic and antioxidant components from natural products within the modern day medical market. The above benefits show that supercritical carbon dioxide extracts of pitaya (H. polyrhizus and H. undatus) peel may very well be a prospective source of compounds with cytotoxic and antioxidant activities as well as the final results give a reference point for additional research on the chemical components of supercritical carbon dioxide extracts of pitaya peel at the same time as for their utilization. Materials and methodsGeneral procedures and reagentsin July 2013. Voucher specimens were deposited at Guizhou Fruit Institute, Guiyang, China.Supercritical carbon dioxide extractionAbout 250 g of dried peel of pitaya (H. polyrhizus and H. undatus) had been reduce into pieces and submitted to extraction. A CO2 flow price of 30 L/h and an extraction period of 60 min had been made use of. The extraction was performed under a pressure of 30 MPa and at a temperature of 40 . The two extracts obtained by supercritical carbon dioxide extraction assay have been pale yellowish. These extracts had been dried over anhydrous Na2SO4 and placed at a low temperature inside the refrigerator until analysis.Gas chromatography-mass spectroscopy (GC-MS) analysisThe melting points with the merchandise had been determined employing an XT-4 binocular microscope (Beijing Tech Instrument Co. Ltd., Beijing, China). 13C NMR were recorded using a JEOL-ECX500 spectrometer at 22 , with tetramethylsilane because the internal common and CDCl3 as the solvent. Column chromatography was performed employing silica gel (20000 meshes) (Qingdao Marine Chemistry Co., Qingdao, China) and Sephadex LH-20 (GE Healthcare Bio-Sciences AB, Uppsala, Sweden). Sodium dodecyl sulfate (SDS) have been bought from Beijing CYP2 Inhibitor Source Dingguo CO., Ltd; 2,2.