He experiment along with the extract was administered as single dose and
He experiment plus the extract was administered as single dose and observed for the mortality up to 48 h study period (short term toxicity). Depending on the quick term toxicity profile, the next dose with the extract was determined as per OECD guidelines No.420. The maximum dose tested (2000 mg/kg) for LD50. In the LD50, doses like 1/20th, 1/10th and 1/5th have been selected and regarded as low, medium and higher dose i.e., one hundred mg/kg, 200 mg/kg, 400 mg/kg respectively to carry out this study.Experimental DesignThe diuretic activity of alcoholic extract of roots of Cissampelos pareira in albino rats was studied by the Lipschitz Test [16-18]. Male Albino rats had been divided into 5 groups of 6 rats in every. The group I serves as standard control received vehicle (CMC 2 in typical saline ten ml/kg b.wt), the group II received Furosemide (10 mg/kg, p.o) in automobile; other groups III, IV, V were treated with low, medium, and high doses of alcoholic extract of roots of Cissampelos pareira in automobile and right away right after the extract remedy all of the rats had been hydrated with saline (15 ml/kg) and placed inside the metabolic cages (two per cage), specially developed to separate urine and faeces andS. no. 1 two 3 4 five groups Control (10 ml/Kg b. wt) Normal (Frusemide 10 mg/kg b.wt) Alcoholic extract of roots of C.pareira Low (one hundred mg/kg b.wt) Alcoholic extract of roots of C.pareira Medium (200 mg/kg b.wt) Alcoholic extract of roots of C.pareira High (400 mg/kg b.wt)DISCUSSIONMedicinal plants and botanicals supply a organic safeguard against illnesses and are a substantial therapy for particular ailments. Diuretics have proved to become very worthwhile in the remedy of mild to moderate hypertension as well as in enhancing the effect of other antihypertensive agents. Diuretics relieve pulmonary congestion and peripheral oedema. These MCT1 drug agents are helpful in lowering volume over load and relieve orthopnea and paroxysmal nocturnal dyspnoea [19] in CCF and acute left ventricular failure. They lower plasma volume and subsequently venous return to the heart. This decreases the cardiac function load, oxygen demand and plasma volume and also decreases blood stress. Thusna+ mmol/l 113.03 + two.16 191.05+2.09 129.40+2.*** ***total urine Vol (ml/kg b.wt/5 h) 13.45.02 22.23.01 15.20.*** ***K+ mmol/l 51.09 + 1.51 87.81+1.60 64.13+1.*** ***Cl- mmol/l 82.95 + 1.42 129.06+1.67*** 94.42 + 1.73*** 109.44+1.20*** 121.39+2.00***17.41.02*** 20.46.***164.99+2.00*** 184.53+2.***77.93+2.67*** 85.11+1.***[Table/Fig-1]: Impact of alcoholic extract of roots of Cissampelos pareira on urine volume and electrolyte concentration in hydrated rat model in albino rats Values expressed as mean S.E.M.,n=6, Significance at p0.05*, p0.01**, p0.001***, Compared with control group (1 Way ANOVA followed by Dunnetts `t’ test).Journal of Clinical and Diagnostic Research. 2014 May well, Vol-8(five): HC01-HCjcdr.netSuresh Babu Sayana et al., Evaluation of Diuretic Activity of Alcoholic Extract of Roots of Cissampelos Pareira in Albino Ratssaponins, organic acids [1,17], steroids, carbohydrates, tannins, phenolic compounds, terpenoids [22], alkaloids [23], glycosides [24], sterols [25], sesquiterpenes aminoacids, carotinoids [26] in distinctive plant CDK3 custom synthesis extracts. Alcoholic extract of roots of Cissampelos pareira was identified with the majority of these plant phytochemical substances described above. Hence it could be reported that the observed diuretic activity is on account of these above phytoconstituents.CONCLUSIONResults showed that single dos.