The extraction. Phytochemical evaluation for the extract was performed using regular
The extraction. Phytochemical evaluation for the extract was performed utilizing normal procedures.kept at 21 .five . The total volume of urine collected for five hr was measured at the finish. For the duration of this period no meals and water was created accessible to animals. Different parameters like total urine volume and concentration of sodium, potassium and chloride inside the urine had been measured and estimated respectively.Estimation of Urinary ElectrolytesUrine electrolytes (sodium, potassium and chloride) have been determined by Ion Selective Electrode technique as described by the user instruction manual of the biochemical kits (Roche, Roche Diagnostics Pvt. Ltd, Gurgaon, Haryana.)Experimental AnimalsAlbino rats weighing amongst 140-200 g of either sex have been employed inside the study and have been obtained from the Central Animal Property, V.L.College of Pharmacy, Raichur, Karnataka, India. The experimental protocol was approved by the Institutional Animal Ethical Committee and these animals had been utilised to evaluate the diuretic activity of alcoholic extract of roots of Cissampelos pareira. The animals had been maintained beneath standard husbandry conditions for an acclimatization period of 15 days prior to performing the experiments. All rats have been housed in metallic cages 6 in each and temperature maintained at 22+2 .STATISTICAL ANALYSISExperimental final results had been expressed as imply + SEM (n=6). Statistical evaluation was performed with one-way-ANOVA followed by Dunnetts ALK3 custom synthesis t-test.RESULTSThe alcoholic extract of roots of Cissampelos pareira was subjected to qualitative phytochemical tests to recognize the phytoconstituents and it revealed the presence of carbohydrates, alkaloids, sterols, phenolic compounds, tannins, flavonoids and resins. In acute toxicity study all the animals were survived even immediately after 14 days. This indicates that the extract was discovered to become protected up to the maximum dose level tested (2000 mg/kg). No key behavioural alterations have been observed in the course of this period of study. The outcomes obtained with evaluation of diuretic activity of alcoholic extract of roots of Cissampelos pareira was shown in [Table/Fig1-3]. From the result it may be observed that alcoholic extract of roots of Cissampelos pareira has shown a important diuretic activity by increasing urinary output and improved excretion of sodium, potassium, chloride when compared to manage. The effect of alcoholic extract of roots of Cissampelos pareira was discovered to become dose dependent, i.e., among the 3 doses studied, greater dose developed far more effect. A comparison was created together with the standard diuretic drug furosemide, the diuretic impact observed after therapy with alcoholic extract of roots of Cissampelos pareira was located to become important in terms of urinary output, sodium, potassium, chloride concentrations. Determination of urinary electrolyte concentration revealed that alcoholic extract of roots of Cissampelos pareira was powerful in growing urinary electrolyte concentrations for all the three ions tested (Na+, K+, Cl-).EthicsThe experiment compiled with all the suggestions for animal experimentation of our laboratory and was approved by the Institutional Animal Ethical Committee (IAEC). Drugs employed Furosemide 20 mg/ml (Sanofi Aventis, Akt1 medchemexpress Andheri East, Mumbai.)Acute toxicity studydetermination of ld50: The acute toxicity [14,15] of alcoholic extract of roots of Cissampelos pareira was determined by using albino mice of either sex (16-20 g), maintained beneath common husbandry situations. The animals had been fasted for three h prior to t.