Ion is essential so as to receive desirable phenolic constituents. Normally, aqueous alcohol (80 methanol and 70 ethanol) are the most preferred solvents to extract phenolic compounds from plants especially herbs [40,41]. Table 1 shows the yield of extracts/fractions and their respective total phenolic content material. The highest volume of phenolic compounds (p 0.05) was discovered in the ethyl acetate fraction which was 1.09 ?0.11 mg of GAEs/g extract, followed by the crude methanol extract (0.75 ?0.07 mg of GAEs/g extract), water fraction (0.61 ?0.02 mg of GAEs/g extract) and hexane fraction (0.25 ?0.03 mg of GAEs/g extract). This result suggested that extractionTable 1 Extraction yields and content material of phenolic compounds in the crude and fractionated extracts of Alpinia pahangensisExtract/fractions Crude methanol Hexane Ethyl acetate Water Weight of extracts (g) 31.19 1.87 2.70 24.43 Total phenolic content (mg/g) 0.75 ?0.07c 0.25 ?0.03a 1.09 ?0.11d 0.61 ?0.02bThis approach has been broadly applied to evaluate the radical scavenging capability in the plant extracts since it is straightforward and extremely sensitive. DPPH, a nitrogen-centered radical having a maximum absorption at 520 nm accepts an electron from an antioxidant which acts as a hydrogen donor. The scavenging activity in the extract was monitored based on the amount of DPPH radicals remaining in the test sample utilizing a spectrophotometer. In our study, the highest scavenging effect was observed inside the ethyl acetate fraction with an IC50 of 0.349 ?0.009 mg/ml. That is followed by the crude methanol extract (0.579 ?0.017 mg/ml), water fraction (0.999 ?0.038 mg/ml) and hexane fraction (2.677 ?0.094 mg/ml). Even so, BHA and ascorbic acid exhibited improved scavenging potential than the ethyl acetate fraction. Table 2 shows the IC50 values from the crude extract and its fractions as in comparison to the standards, BHA and ascorbic acid.Determination of lowering powerFigure 1 shows the Met Inhibitor web Reductive potential with the crude and fractionated extracts in the rhizomes of A. pahangensis in comparison to BHA and ascorbic acid. Reductive ability was measured by the reduction of ferricyanide complex/Fe3+ to the ferrous type (Fe2+) in the presence of antioxidant (reductant). The Fe2+ formation produce Perl’s Prussian blue and can be monitored at absorbance of 620 nm by a spectrophotometer. The reductive capability in the extracts and the regular compounds enhanced inside the following order: water hexane ethyl acetate methanol BHA ascorbic acid. The decreasing power with the extract increased with all the improve in concentration with the extract until it reaches a particular level and then turn out to be constant. Essentially, reducing energy isTable 2 Radical scavenging activity in the crude and fractionated extracts of Alpinia pahangensis against DPPH radicalExtracts and requirements Crude methanol Hexane Ethyl acetate Water Ascorbic acid BHA IC50 values (mg/ml) 0.579 ?0.017 c two.677 ?0.094 e 0.349 ?0.009 b 0.999 ?0.038 d 0.015 ?0.600 a 0.013 ?0.600 aValues expressed are imply ?SD of triplicate measurements. Means with distinct β adrenergic receptor Modulator custom synthesis letters (a-d) within the identical column are substantially various (p 0.05). mg/g: mg of gallic acid equivalents/g of extract or fractions.IC50 values expressed are mean ?common deviation of triplicate measurements. Signifies with various letters (a-e) within the similar column are drastically unique (p 0.05). BHA and Ascorbic acid had been utilized as standards.Phang et al. BMC Complementary and Alternative Medicine 2013, 13:243 biomedcentral/14.