Stiansen EN, Lund JS, Rortveit T, Rustan AC: Effect of dietary n-3 and n-6 fatty acids on fatty acid desaturation in rat liver. Biochim Biophys Acta 1991, 1082:57?2. 46. Igarashi M, Ma K, Chang L, Bell JM, Rapoport SI: Dietary n-3 PUFA deprivation for 15 weeks upregulates elongase and desaturase expression in rat liver but not brain. J Lipid Res 2007, 48:2463?470. 47. Tu WC, Cook-Johnson RJ, James MJ, Muhlhausler BS, Gibson RA: Omega-3 long chain fatty acid synthesis is regulated much more by substrate levels than gene expression. Prostaglandins Leukot Essent Fatty Acids 2010, 83:61?eight.doi:ten.1186/1476-511X-12-147 Cite this article as: Casey et al.: Effect of stearidonic acid-enriched soybean oil on fatty acid profile and metabolic parameters in lean and obese Zucker rats. Lipids in Health and Illness 2013 12:147.
THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 290, NO. three, pp. 1319 ?331, January 16, 2015 ?2015 by The American Society for Biochemistry and Molecular Biology, Inc. Published in the U.S.A.Involvement from the Na /Ca2 exchanger isoform 1 (NCX1) in neuronal Development Factor (NGF)-induced Neuronal Differentiation via Ca2 -dependent Akt PhosphorylationReceived for publication, July 7, 2014, and in revised type, November 21, 2014 Published, JBC Papers in Press, November 21, 2014, DOI 10.1074/jbc.M114.Agnese Secondo1, Alba NK1 Modulator Storage & Stability Esposito1, Rossana Sirabella?, Francesca Boscia, Anna Pannaccione, Pasquale Molinaro, Maria Cantile, P2X7 Receptor Inhibitor supplier Roselia Ciccone, Maria Jos?Sisalli, Antonella Scorziello, Gianfranco Di Renzo, and Lucio Annunziato? In the Department of Neuroscience and Reproductive and Odontostomatological Sciences, School of Medicine, “Federico II” University of Naples, Via Sergio Pansini 5, Naples 80131, Italy as well as the �Fondazione Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) SDN, Naples 80143, ItalyBackground: NCX1 regulates intracellular Ca2 and Na homeostasis in neurons. Final results: The overexpression of NCX1 induced neuronal differentiation by way of Akt also as NGF exposure. NCX1 knockdown prevented NGF-induced neurite outgrowth. Conclusion: NCX1 participates in neuronal differentiation by ionic regulation and Akt phosphorylation. Significance: Finding out how NCX1 participates in neurite outgrowth will increase the know-how of neuronal differentiation. NGF induces neuronal differentiation by modulating [Ca2 ]i. However, the role of your three isoforms with the major Ca2 -extruding system, the Na /Ca2 exchanger (NCX), in NGF-induced differentiation remains unexplored. We investigated irrespective of whether NCX1, NCX2, and NCX3 isoforms could play a relevant part in neuronal differentiation via the modulation of [Ca2 ]i plus the Akt pathway. NGF triggered progressive neurite elongation; a significant increase of your well known marker of development cones, GAP-43; and an enhancement of endoplasmic reticulum (ER) Ca2 content material and of Akt phosphorylation through an early activation of ERK1/2. Interestingly, throughout NGF-induced differentiation, the NCX1 protein level improved, NCX3 decreased, and NCX2 remained unaffected. At the identical time, NCX total activity increased. In addition, NCX1 colocalized and coimmunoprecipitated with GAP-43, and NCX1 silencing prevented NGF-induced effects on GAP-43 expression, Akt phosphorylation, and neurite outgrowth. Alternatively, the overexpression of its neuronal splicing isoform, NCX1.4, even inside the absence of NGF, induced an increase in Akt phosphorylation and GAP-43 protein expression. Interestingly, tetrodotoxin-sensitive Na curren.