Tenoid [14]. We analyzed I1527cm-1 between typical and malignant gastric tissues with Two Independent Sample Annexin V-FITC/PI Apoptosis Detection Kit custom synthesis t-Test then CDCP1 Protein Species discovered that the relative intensity with the peak I1527 cm-1 was drastically stronger in cancer tissue than in normal tissue (p,0.05), indicating that carotenoid content in cancer tissue is improved. Carotenoid consisted of carotene, carotol, propane diacid and so on. Human tissues contained carotene mostly, includingb- carotene, acarotene,c- carotene and so on. b- carotene is definitely an antioxidant that will guard proteins and nucleic acids from damage by totally free radicals and cut down the harm to genetic material plus the cell membrane. The considerable enhance of Carotenoid in cancer cells suggests that cancer cells probably evolve an enhanced capability to resist harm. As well as its antioxidant function, carotenoid is involved inside the synthesis of glycoproteins in vivo. The proliferation and differentiation of normal gastrointestinal epithelial cells require retinoic acid, indicating that cancer cells may well really need to synthesize a lot more glycoproteins than normal cells and that metabolism in cancer cells may be a lot more active than in typical cells. Compared with standard tissue, the peak representing the ringbreathing vibration in the indole ring of tryptophan in the cancer tissue spectrum shifted from 758 cm-1 to 759 cm-1, “blue shift” occured. These outcomes recommend that the structure of tryptophan is additional stable in cancer tissue or its elevated stabilizition was impacted by the activation from the neighboring functional groups. It may indicates that far more tryptophan is situated within a hydrophobic environment, like the core of globin [20]. Our results also indicate that the selection of protein species along with the conformation of proteins are changed in cancer tissues. Inside the spectrum of cancer tissue involving 1338 and 1447 cm-1, a peak representing unsaturated fatty acids appeared at 1379 cm-1 that was absent within the spectrum of standard tissue. The relative intensity with the peak representing unsaturated fatty acids at 1585 cm-1, I1585 cm-1, was significantly increased in cancer tissue compared with normal tissues (Two Independent sample t-Test, p,0.05), suggesting that the content of unsaturated fatty acids in cancer tissue is improved. Cell membrane mobility is positively correlated together with the content material of fatty acids in cells. The improve of unsaturated fatty acid content in cancer cells suggests that cancer cell membrane mobility increases, which facilitates the transportation and metabolism of transmembrane molecules. Compared with normal tissue, the peak at 938 cm-1 shifted to 944 cm-1 in cancer tissue, a “blue shift”, indicating that the energy of vibration increased. This peak is attributed to the stretching vibrations of proline and valine [24] and represents the a helix of collagen. This result indicates a conformational alter in collagen structure in cancer tissue; things contributing to a peak shift consist of activation, adhesion, and twisting of functional groups. Additional with the a helix may be exposed, activated, and formed to improve the vibration. Even so, the relative intensity of I1585cm1/I853cm-(854 cm-1) in cancer tissues was significantly stronger than that of typical ones (Two Independent sample t-Test, p,0.05), indicating that collagen content in cancer tissue is substantially lowered. Cancer cells synthesize and secrete matrix metalloproteinases to degrade matrix proteins including collagen, facilitating cancer met.