Product Name :
Ubiquitin-AMC
Sequence:
Purity:
≥95% (HPLC)
Molecular Weight:
Solubility :
Appearance:
Use/Stability :
As indicated on product label or CoA when stored as recommended.
Description:
High purity industry standard substrate for deubiquitinylating enzymes (DUBs). Ubiquitin-AMC is a fluorogenic substrate for a wide range of deubiquitinylating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin specific proteases (USPs). It is a particularly useful reagent for the study of deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential.85622-93-1 MedChemExpress Ubiquitin-AMC has been shown to be a sensitive substrate for UCH-L3 and for Isopeptidase-T , and is particularly useful for studying deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential. Figure: DUB Assay: 50nM UCLH1, His6-tagged (UW9740); 500nM Ubiquitin-AMC; 50mM HEPES pH7.8, 0.5mM EDTA, 1mM DTT. Figure: HPLC analysis. Comparison of Prod. No. BML-SE-211Prod. No. BML-SE-211) (A) with contaminated alternatively sourced material (B). Figure: DUB Assay: 50nM UCLH1, His6-tagged (UW9740); 500nM Ubiquitin-AMC; 50mM HEPES pH7.8, 0.5mM EDTA, 1mM DTT.2099142-76-2 web Figure: HPLC analysis.PMID:29999663 Comparison of Prod. No. BML-SE-211Prod. No. BML-SE-211) (A) with contaminated alternatively sourced material (B).
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Solubility:
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Additional Information :
| Application Notes Can be used for the following applications:1. Substrate for deubiquitinylating enzyme activity assays.2. Identification/confirmation of enzyme deubiquitinylation activity.3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL substrates. | Formulation In DMSO | Purity ≥95% (HPLC) | Purity Detail Please note: considerable efforts are taken to ensure that ubiquitin-AMC is homogeneous and not contaminated with ubiquitin and ubiquitin deletions, which are common in material obtained from other sources. The chromatogram below demonstrates the homogeneity of BML-SE211. | Source Produced in E.coli. | UniProt ID P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52)