L activity. Tesaglitazar web Conclusions: The substantial aggregates of gliadins that can take place throughout bread-making displayed a decreased allergenicity in vitro in comparison to native gliadins. This may perhaps be connected to the capacity of some sufferers to attain hypo-responsiveness to wheat through oral immunotherapy protocols performed with bread or other heated wheat-based products. P13 Scavenger receptor class a mediates uptake of Ara H 1, a significant peanut allergen, by human M2 macrophages Maren Krause1, Peter Crauwels2, Martin Globisch3, Thomas Henle3, Ger Van Zandbergen2, Stefan Vieths1, Stephan Scheurer1, Masako Toda1 1 VPr1 Investigation Group “Molecular Allergology”, PaulEhrlichInstitut, Langen, Germany; 2Division of Immunology, PaulEhrlichInstitut, Langen, Germany; 3Institute of Meals Chemistry, Technical University Dresden, Dresden, Germany Correspondence: Maren Krause [email protected] Clinical Translational Allergy (CTA) 2018, eight(Suppl 1):P13 Background: Ara h 1 potentially contributes to peanut-induced anaphylactic reactions as a major peanut allergen. Dendritic cell-specific ICAM-grabbing nonintegrin (DC-SIGN) is described as receptor for Ara h 1 to activate human dendritic cells (Shreffler et al., J. Immulol. 2006), whereas Ara h 1-mediated activation of macrophages is less investigated. Considering that evidence has accumulated that not just dendritic cells but also macrophages play a vital part in development and upkeep of meals allergy, we aimed to investigate interaction of Ara h 1 with human principal macrophages. Methods: M1 and M2 macrophages were generated by culturing peripheral blood derived monocytes from wholesome donors in the presence of rGM-CSF and rM-CSF for 6-8 days, respectively. Ara h 1 was isolated from unroasted peanut. Levels of Ara h 1 uptake and receptor expression by macrophages had been assessed by flow cytometry. The levels of secreted cytokines by Ara h 1-stimulated cells had been assessed by ELISA. Interaction of Ara h 1 with receptors expressed on the cell surface of macrophages was investigated employing inhibitors of putative cell surface receptors and compact interfering RNA.Clin Transl Allergy 2018, eight(Suppl 1):Page 6 ofResults: Upon stimulation with Ara h 1, M1 macrophages made higher levels of pro-inflammatory cytokines IL-6 and TNF- than M2 macrophages. In contrast, M2 macrophages internalized Ara h 1 to a higher extent than M1 macrophages. M1 Saccharin sodium Biological Activity macrophage expressed DC-SIGN and SR-A only at marginal levels, whereas M2 macrophages expressed both receptors at considerable levels. Modest interfering RNA knockdown of DC-SIGN in M1 and M2 macrophages did not suppress the uptake of Ara h 1 by the cells. Nevertheless, inhibitors for scavenger receptor class A (SR-A), e.g. polyinosinic acid and acetylated low density lipoprotein, suppressed M2 macrophage-mediated, but not M1 macrophage-mediated uptake of Ara h 1. Conclusions: Within this study, we demonstrated that DC-SIGN is probably to not be a major receptor involved in the interaction of Ara h 1 by human primary macrophages. SR-A is demonstrated to partly mediate the interaction of Ara h 1 with M2 macrophages, which play an active role in the pathogenesis of allergy. Additional research are essential to obtain a deeper understanding of your interaction amongst Ara h 1 and M2 macrophages and to unravel the mechanism underlying the intrinsic allergenicity. P14 Genetic variation influences the impact of PGE2 on allergic responses in murine mast cells Shruti Rastogi, Maria Nassiri, Magda Babina, Margitta Worm AllergyCen.