He experiment along with the extract was administered as single dose and
He experiment along with the extract was administered as single dose and observed for the mortality as much as 48 h study period (quick term toxicity). Determined by the brief term toxicity profile, the next dose with the extract was determined as per OECD guidelines No.420. The maximum dose tested (2000 mg/kg) for LD50. From the LD50, doses like 1/20th, 1/10th and 1/5th have been chosen and considered as low, medium and higher dose i.e., one hundred mg/kg, 200 mg/kg, 400 mg/kg respectively to carry out this study.Experimental DesignThe diuretic activity of alcoholic extract of roots of Cissampelos pareira in albino rats was studied by the Lipschitz Test [16-18]. Male Albino rats have been divided into 5 groups of six rats in every single. The group I serves as typical manage received vehicle (CMC 2 in typical saline 10 ml/kg b.wt), the group II received Furosemide (10 mg/kg, p.o) in vehicle; other groups III, IV, V had been treated with low, medium, and higher doses of alcoholic extract of roots of Cissampelos pareira in vehicle and promptly immediately after the extract therapy all the rats have been hydrated with saline (15 ml/kg) and placed within the metabolic cages (2 per cage), specially developed to separate urine and faeces andS. no. 1 2 3 four five groups Handle (10 ml/Kg b. wt) Regular (Frusemide ten mg/kg b.wt) Alcoholic extract of roots of C.pareira Low (one hundred mg/kg b.wt) Alcoholic extract of roots of C.pareira Medium (200 mg/kg b.wt) Alcoholic extract of roots of C.pareira Higher (400 mg/kg b.wt)DISCUSSIONMedicinal plants and botanicals provide a all-natural safeguard against diseases and are a substantial treatment for particular ailments. Diuretics have proved to become extremely valuable in the treatment of mild to moderate hypertension and also in enhancing the impact of other antihypertensive agents. Diuretics relieve pulmonary congestion and peripheral oedema. These agents are beneficial in ACAT2 Biological Activity minimizing volume over load and relieve orthopnea and paroxysmal nocturnal dyspnoea [19] in CCF and acute left ventricular failure. They lower plasma volume and subsequently venous return for the heart. This decreases the cardiac operate load, oxygen demand and plasma volume and also decreases blood pressure. Thusna+ mmol/l 113.03 + two.16 191.05+2.09 129.40+2.*** ***total urine Vol (ml/kg b.wt/5 h) 13.45.02 22.23.01 15.20.*** ***K+ mmol/l 51.09 + 1.51 87.81+1.60 64.13+1.*** ***Cl- mmol/l 82.95 + 1.42 129.06+1.67*** 94.42 + 1.73*** 109.44+1.20*** 121.39+2.00***17.41.02*** 20.46.***164.99+2.00*** 184.53+2.***77.93+2.67*** 85.11+1.***[Table/Fig-1]: Effect of alcoholic extract of roots of Cissampelos pareira on urine volume and electrolyte concentration in hydrated rat model in albino rats Values expressed as imply S.E.M.,n=6, Significance at p0.05*, p0.01**, p0.001***, Compared with manage group (1 Way ANOVA followed by Dunnetts `t’ test).Journal of Clinical and Diagnostic Research. 2014 May perhaps, Vol-8(5): HC01-HCjcdr.netSuresh Babu Sayana et al., Evaluation of Diuretic Activity of Alcoholic Extract of Roots of Cissampelos Pareira in Albino Ratssaponins, organic acids [1,17], steroids, carbohydrates, tannins, phenolic compounds, MEK1 medchemexpress terpenoids [22], alkaloids [23], glycosides [24], sterols [25], sesquiterpenes aminoacids, carotinoids [26] in various plant extracts. Alcoholic extract of roots of Cissampelos pareira was identified with most of these plant phytochemical substances talked about above. Hence it can be reported that the observed diuretic activity is due to these above phytoconstituents.CONCLUSIONResults showed that single dos.